Zoe and Dolores are the sisters of Desmond Belmont and vampire hunters all the same. As was their brother, they, too, were victim of their parents' murder at the hands of Rohan Krause and were left to fend for themselves. Without much in the way of guidance, the three grew together with the closest of bonds, unaware of their parents' true murderer (the elders kept from them Krause's true identity in the hope that anger would play no role in their development and potentially outweigh their sense of justice).

In the trio's bid to discover the link between The Order and the forces of Count Dracula, it was the sisters who handled most the investigation of the villain's mansion while Desmond took to clearing the area in search of the Sacrificial Altar, where Rohan Krause was in the final stage of preparation for Dracula's resurrection. It was they who uncovered an enchanted gauntlet, which according to family confidant Giovanni was an alchemy-based artifact tied to the Belmonts' history, and rushed it over to Desmond, who could then use its magical properties to harness deadly spells. They also deciphered the castle's interconnected nature, which allowed for Desmond to locate the Inner Sanctum, a separate entity where the altar, and more importantly Krause, was located.

Zoe was the more talkative of the two sisters and kept more closely in touch with Desmond during his campaign. It was she who checked on him after his first boss encounter; she also brought him the gauntlet and relayed to him the sisters' discoveries. Dolores, the youngest of the trio and the most business-minded, preferred to remain hard at work scouring the castle in search of clues; Dolores was said to be equally as capable of wielding the Vampire Killer but was not yet of age.

Though they soon after Krause's defeat left the sanctum to head back to the mansion to investigate further (more so to leave the confrontation with Krause in Desmond's capable hands), they turned back just in case. Their fears unfounded--Krause and then even Dracula having been defeated--they arrived in time to witness Desmond's finishing off the Dark Lord with a mighty whip-slash. Their only concern was the safety of their brother, and of that they were assured. Standing together, as they always had, the three escaped the castle and headed home.